posted on 2016-03-21, 05:00authored byTimothy Swain, Emily DuBois, Andrew Gomes, Valentina Stoyneva, Andrew Radosevich, Jillian Henss, Michelle Wagner, Justin Derbas, Hannah Grooms, Elizabeth Velazquez, Joshua Traub, Brian Kennedy, Arabela Grigorescu, Mark Westneat, Kevin Sanborn, Shoshana Levine, Mark Schick, George Parsons, Brendan Biggs, Jeremy Rogers, Vadim Backman, Luisa Marcelino
Additional file 9: Figure S6. Experimental setup. High temperature aquarium encompassing the HT-CL and HT-HL conditions on day 11 of the bleaching experiment (a); black divider separating light arrays, flow baffles, and mounted corals can be seen. Collecting PAM measurements in the control temperature aquarium within the CT-CL condition (CT-HL on the opposite side of black divider) on day 11 of the bleaching experiment (b); positioning of the PAM instrument probes above the coral explants mounted on stone tiles can be seen. Close up of probe holder (custom-machined acrylic block that ensures probes are returned to each explant in the same three-dimensional geometry as previous measurements) supported at a 23° angle by square PVC post (gray) attached to the coral-mounting tile (c); probes are (left to right) temperature, O2 (data not reported), and PAM fiber optic immobilized in a black PVC tube. Control temperature aquarium encompassing the CT-CL and CT-HL conditions (HT aquarium in background) during acclimation period prior to prescreening and fragmentation (d); photograph taken before installation of the flow baffles and black divider separating light arrays. Hand-held optical fiber attached to a spectrometer to measure R H (and R S of cleaned skeleton) with white reflectance standard visible in the background (e). Consent to publish these images has been documented.