13568_2019_772_MOESM1_ESM.docx (457.59 kB)
MOESM1 of A novel method for the capture-based purification of whole viral native RNA genomes
journal contribution
posted on 2019-04-08, 05:00 authored by Cedric Tan, Sebastian Maurer-Stroh, Yue Wan, October Sessions, Paola de SessionsAdditional file 1: Table S1. Reagents and volumes for hybridization step. Table S2. Reagents and volumes for RT-qPCR. Table S3. RT-qPCR program set on Applied Biosystems ViiA 7 Real Time-PCR System. Table S4. Primers, probes and standards used with the respective modifications for RT-qPCR. Table S5. Reagents and volumes used for polyadenylation of RNA samples. Figure S1. DENV1 and GAPDH standard curves used for calculation of primer efficiency. Figure S2. Coverage plot against nucleotide position on DENV1 reference for pre-capture MinION sequencing run. Figure S3. Coverage plot against nucleotide position on DENV1 reference for post-capture MinION sequencing run. Figure S4. Coverage plot against nucleotide position on DENV1 reference for concentrated post-capture MinION sequencing run.