12951_2020_680_MOESM1_ESM.docx (88.5 kB)
Additional file 1 of Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer
journal contribution
posted on 2020-08-28, 03:59 authored by Yi-An Cheng, Tung-Ho Wu, Yun-Ming Wang, Tian-Lu Cheng, I-Ju Chen, Yun-Chi Lu, Kuo-Hsiang Chuang, Chih-Kuang Wang, Chiao-Yun Chen, Rui-An Lin, Huei-Jen Chen, Tzu-Yi Liao, En-Shuo Liu, Fang-Ming ChenAdditional file 1: Table S1. The BsAb-conjugation rate of αHER2/PEG-NPs. Table S2. The Characterization of BsAb/mPEG-NPs. Figure S1. Immunogenicity of humanized BsAbs. We cocultured dendritic cells differentiated from human PBMCs with autologous CD4+ T cells and stimulated with control medium (represented as DC+T), PHA (as positive control), PEG×HER2, PEG×DNS, respectively, for 5 days. Then, we detected the proliferation of CD4+ T cells by ATPlite assay. Bars, SD. CPM, counts per minute; PBMC, peripheral blood mononuclear cell; PHA, phytohemagglutinin.
