MOESM8 of Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken

Additional file 8. Frequencies of IFN-γ-producing CD4+ and CD4−CD8β− cells in the liver. The upper panel shows frequencies of IFN-γ-producing CD4+ and CD4−CD8β− cells after PMA/ionomycin or H. meleagridis/E. coli stimulation compared to medium or E. coli-only stimulation in control and infected birds. The lower panel compares frequencies of IFN-γ-producing CD4+ and CD4−CD8β− cells after stimulation with PMA/ionomycin, H. meleagridis/E. coli or after correction for E. coli between infected and control birds. Each symbol represents one bird, black and red colored symbols show birds sacrificed 2 weeks pi and 5 weeks pi, respectively, as percent of total CD4+ or CD4−CD8β− intrahepatic lymphocytes. Asterisks indicate p-value: *p ≤ 0.05.