MOESM7 of Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken

Additional file 7. Influence of different H. meleagridis concentrations on the frequency of IFN-γ-producing CD4+ splenocytes. Intracellular cytokine staining for IFN-γ was performed following 18 h antigen specific re-stimulation either with H. meleagridis at 5 × 104/mL and E. coli (9.4 × 106 CFU/mL) or a 10-fold lower concentration of H. meleagridis (5 × 103/mL) and E. coli (9.4 × 105 CFU/mL). Plots on the left of each stimulation variant compare frequencies of IFN-γ-producing CD4+ cells after combined H. meleagridis/E. coli stimulation or stimulation only with E. coli in infected and control chickens. Plots on the right compare frequencies of IFN-γ-producing CD4+ cells between infected and control chickens after stimulation with H. meleagridis/E. coli antigen with or without correction for the response against E. coli alone. Each symbol represents one bird, black and red colored symbols show birds sacrificed 2 weeks pi and 5 weeks pi, respectively, as percent of total CD4+ splenocytes. Asterisks indicate different p-values: *p ≤ 0.05, and **p ≤ 0.01.