MOESM2 of Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts

Additional file 2: Supplementary figures, including Figure S1. Characterization of EVs; Figure S2. Representative images of the distribution of EVs from SACC-LM, CAF-A1 and CAF-A2 cells in the lungs, liver and brain of mice; Figure S3. ECM remodeling in the lungs at days 7 and 14 after EV treatment; Figure S4. EVs were taken up by specific cell types in the lungs; Figure S5. Phenotype changes were examined in lung fibroblasts treated with indicated EVs; Figure S6. LFs were not activated by NF EVs; Figure S7. Integrin β1 expression functionally contributed to CAF EV uptake by LFs in vitro and mediated pre-metastatic niche formation in the lungs; Figure S8. Cytotoxicity assays of TC I-15; Figure S9. Lung examination to confirm the pre-metastatic niche formation in the nude mice with indicated xenografts for 3 weeks; Figure S10. Xenograft examination after subcutaneous transplantation for 5 weeks; Figure S11. Lung examination after subcutaneous transplantation for 5 weeks; Figure S12. Mouse plasma EV characterization.