MOESM1 of Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment

Additional file 1: Fig. S1. The DOE study results were analyzed by JMP statistical software custom Design-Fit least square modeling. The Prob > F value, under the Analysis of Variance is the P value for the model, along with R2 value of the model, 0.0091 and 0.929494, suggest a significant model. Model analysis indicates that a shift in temperature from 37 to 32 °C along with a combination of DO and temperature shift have a significant effect on rFab production as indicated by the Probability  > |t| values of 0.0044 and 0.0250, respectively. Fig. S2. SDS-PAGE analysis of rFabs from each of the eight bioreactors in the DOE Optimization study under non-reduced condition. The SDS-PAGE samples were collected at day eighteen of the cell culture. Lane M is molecular weight markers. Lanes 1 through 8 represent bioreactors 1 through 8.