Additional file 9: of Lysophosphatidic acid receptor 1 (LPA1) plays critical roles in microglial activation and brain damage after transient focal cerebral ischemia

Figure S9. LPA1 antagonism alters expression levels of few anti-inflammatory cytokines in the ischemic brain after tMCAO challenge. Mice were challenged with tMCAO. AM095 (30 mg/kg, p.o.) was administered immediately after reperfusion. Total RNA was extracted from the ipsilateral brain hemisphere at 1 day and 3 days after tMCAO challenge, and mRNA expression levels of anti-inflammatory cytokines were determined by qRT-PCR analysis. Changes in expression levels of anti-inflammatory cytokines at 1 day (a-c) and at 3 days (d-f) are shown. n = 5 mice per group. ***p < 0.001 versus sham. ##p < 0.01 versus vehicle-administered tMCAO mice (tMCAO+veh). (PPTX 54 kb)