12885_2019_5563_MOESM6_ESM.docx (130.42 kB)
Additional file 6 of Glucocorticoid-dependent expression of IAP participates in the protection against TNF-mediated cytotoxicity in MCF7 cells
journal contribution
posted on 2019-04-15, 05:00 authored by Irma Mitre-Aguilar, Tonatiuh Barrios-Garcia, Victor Ruiz-Lopez, Alberto Cabrera-Quintero, Nancy Mejia-Dominguez, Jose Ventura-Gallegos, Daniel Moreno-Mitre, Alejandro Aranda-Gutierrez, Janini Mejia-Rangel, Alma Escalona-Guzman, Yanin Chavarri-Guerra, Alfonso Leon-Del-Rio, Alejandro Zentella-DehesasiRNA against c-IAP1 or XIAP decreased the cytotoxic protection of GCs. Values represent the % viability at 18 h post-treatment. MOCK: Transfection without siRNA. NR-siRNA: Transfection with nonrelated siRNA. c-IAP1-siRNA: Transitory transfection with siRNA targeting c-IAP1 transcripts. XIAP-siRNA: Transitory transfection with siRNA targeting c-XIAP transcripts. The siRNAs selected for this experiment resulted in the lowest expression of their target IAP (as shown in Fig. 6) (*): Represents the % loss of protection in cells with siRNA-mediated inhibited c-IAP1 and XIAP vs cells treated with CORT + TNF + NR-siRNA or DEX + TNF + NR-siRNA. (DOCX 130 kb)
