Additional file 3: of The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer

Figure S3. TSLP mRNA isoform expression in CAFs in steady state conditions or after activation. For activation CAFs (n = 5) were treated with recombinant cytokines (IL-1α, IL-1β and TNF-α), which were used either alone or in combinations at the concentration of 20 ng/ml/each cytokine. a Expression of short and long TSLP forms by CAF untreated (basal) or activated by cytokine treatment (activated). b Fold (DOCX 191 kb) increase in TSLP mRNA expression after activation for the long and short TSLP isoforms. Significance was determined by the Wilcoxon matched-pairs signed rank test (a) and Mann Whitney test (b). Values significantly different were indicated as: p < 0.05. (DOCX 395 kb)