Additional file 1: of Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food

Figure S1. Schemes of one-step indirect ELISA, two-step indirect ELISA and one-step direct ELISA for colistin. Figure S2. Ultraviolet-visible spectrum of four GNPs solutions synthesized by reducing chlorauric acid with 1.0, 1.6, 2.0 and 2.5 mL of citrate solution. Figure S3. TEM images of four GNPs solutions synthesized by reducing chlorauric acid with 1.0 mL (A), 1.6 mL (B), 2.0 mL (C) and 2.5 mL (D) of citrate solution. Table S1. The IC50 values of all sera from mice immunized with two different dosages. Table S2. Comparison of one step ci-ELISA, two step ci-ELISA and one step cd-ELISA regarding IC50 values and assay time (n = 4). Table S3. Comparison of the developed ELISA method with that literature reported enzyme immunoassay. Table S4. Detection results of colistin in actual animal feed by ELISA, LFIA and HPLC method. (DOCX 723 kb)