Additional file 1: of Metformin induces apoptosis in mesenchymal stromal cells and dampens their therapeutic efficacy in infarcted myocardium

Table S1. Demographic and clinical characteristics of patients. Figure S1. Identification of MSCs. A hUC-MSC in normal condition (40×); B osteogenic differentiation of hUC-MSC; C chondrogenic differentiation of hUC-MSC; D adipogenic differentiation of hUC-MSC; E detection of surface markers of hUC-MSC by flow cytometry. Figure S2. Metformin has no effect on mouse cardiomyocytes, human endothelial cells, and human fibroblast survival. A and B, the ratio of mouse cardiomyocyte apoptosis (annexin V+) induced by 2 mM metformin at 24 h, 48 h, and 72 h; C and D, the ratio of human endothelial cells apoptosis (annexin V+) induced by 2 mM metformin at 24 h, 48 h, and 72 h; E and F, the ratio of human fibroblast apoptosis (annexin V+) induced by 2 mM metformin at 24 h, 48 h, and 72 h. Bars in B, D, and F represent the mean ± SEM (n = 3 per group). Statistical test applied by one-way ANOVA. (DOCX 11562 kb)