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Additional file 1 of CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma

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posted on 2020-03-10, 04:43 authored by Simin Shao, Haowei Cao, Zhongkun Wang, Dongmei Zhou, Chaoshen Wu, Shu Wang, Dian Xia, Daoyong Zhang
Additional file 1: Table S1. Primer pairs for real-time PCR. Table S2. Primer pairs for ChIP-QPCR. Figure S1. The CHD4/NuRD complex regulates complement gene expression. Gene Set Enrichment Analysis (GSEA) analysis was performed to identify the pathways altered in high_expression HCC samples compared to low_expression samples for subunits HDAC1, KDM1A, MTA3, RBBP4 and RBBP7 respectively. Figure S2. CHD4 knockdown efficiency was measured in Hep3B cells using western blotting analyses. β-Tubulin was used as loading control.

Funding

National Natural Science Foundation of China Natural Science Foundation of the Jiangsu Higher Education Institutions of China Natural Science Foundation of Jiangsu Province Xuzhou Jinlonghu Distinguished Talents Program Research Foundation of Xuzhou Medical University Xuzhou Science and Technology Innovation Program

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    Clinical Epigenetics

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    NuRDHepatocellular carcinomaImmune infiltrationComplement genes

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