Figure S1. Sequence alignment of the aat gene from different Pediococcus acidilactici strains. The two primers aat_Fw and aat_Rv were used in the PCR reactions. primer_aat_Fw 5'-ATGAGTGATAAAGTTAACGC-3' FAM18098 1 ATGAGTGATAAAGTTAACGCACTATTAGAACGCATCAAACCATCTGTAAAGAATACTCAA 60 FAM17418 79209 ............................................................ 79268 FAM17411 79209 ............................................................ 79268 FAM17422 79209 ............................................................ 79268 FAM13881 253124 ............................................................ 253183 FAM13473 8055 ............................................................ 7996 FAM20559 251239 ............................................................ 251298 DSM 20284 556094 ............................................................ 556153 FAM19460 409021 ............................................................ 409080 FAM18987 526123 ...................................T..G.....C..T..A.....G... 526182 FAM18098 61 ATGGCGCAAGTTTTTAAATACAATGAAGAAATTTCTAAAATCCCAGGAGTAATTAAGCTA 120 FAM17418 79269 ............................................................ 79328 FAM17411 79269 ............................................................ 79328 FAM17422 79269 ............................................................ 79328 FAM13881 253184 ............................................................ 253243 FAM13473 7995 ............................................................ 7936 FAM20559 251299 ............................................................ 251358 DSM 20284 556154 .........................................T.................. 556213 FAM19460 409081 .........................................T.................. 409140 FAM18987 526183 .....A..............T..............A.....T..G.....G......T.. 526242 FAM18098 121 ACCCTTGGAGAACCCGACTTCAATACCCCCGAGCACGTCAAAGAGGCTGGCATTCAAGCC 180 FAM17418 79329 ............................................................ 79388 FAM17411 79329 ............................................................ 79388 FAM17422 79329 ............................................................ 79388 FAM13881 253244 ............................................................ 253303 FAM13473 7935 ............................................................ 7876 FAM20559 251359 ............................................................ 251418 DSM 20284 556214 ................................A..................G........ 556273 FAM19460 409141 ................................A..................G........ 409200 FAM18987 526243 .....C.................C..G.....T...........A............... 526302 FAM18098 181 ATTCGGGATAACAAGAGCCATTACACCGCCACCGCTGGGGACCCCAAAGTTCGTCAAGCG 240 FAM17418 79389 ............................................................ 79448 FAM17411 79389 ............................................................ 79448 FAM17422 79389 ............................................................ 79448 FAM13881 253304 ............................................................ 253363 FAM13473 7875 ............................................................ 7816 FAM20559 251419 ............................................................ 251478 DSM 20284 556274 ............................................................ 556333 FAM19460 409201 ............................................................ 409260 FAM18987 526303 ........C...........C...........T........T..T.....A......... 526362 FAM18098 241 GCAGCCCAATACTTAAAACAAAAATACGATCAAGATTACGATCCAGAAAATGAACTTGTC 300 FAM17418 79449 ............................................................ 79508 FAM17411 79449 ............................................................ 79508 FAM17422 79449 ............................................................ 79508 FAM13881 253364 ..........................................T.............C... 253423 FAM13473 7815 ..........................................T.............C... 7756 FAM20559 251479 ..........................................T.............C... 251538 DSM 20284 556334 ............................G............C..............C..T 556393 FAM19460 409261 ............................G.........T..C..............C..T 409320 FAM18987 526363 ..G.......................T.G.....................C.....C..T 526422 FAM18098 301 ATTACGGCTGGGGTTACCGGTGGAATGTACGCCACCCTAACGTCCATCGTTAACGAAGGT 360 FAM17418 79509 ............................................................ 79568 FAM17411 79509 ............................................................ 79568 FAM17422 79509 ............................................................ 79568 FAM13881 253424 ....................................................G....... 253483 FAM13473 7755 ....................................................G....... 7696 FAM20559 251539 ....................................................G....... 251598 DSM 20284 556394 ....................................................G....... 556453 FAM19460 409321 ....................................................G....... 409380 FAM18987 526423 ..............G..T..G...........T........C......A...G......C 526482 FAM18098 361 GACGAAGTTTTAATACCAACGCCAATTTTTCCATTGTACTTCCCAATCGTTAAAACTAAC 420 FAM17418 79569 ............................................................ 79628 FAM17411 79569 ............................................................ 79628 FAM17422 79569 ............................................................ 79628 FAM13881 253484 ............................................................ 253543 FAM13473 7695 ............................................................ 7636 FAM20559 251599 ............................................................ 251658 DSM 20284 556454 ............................................................ 556513 FAM19460 409381 ............................................................ 409440 FAM18987 526483 ..............T..G..A...........GC.T........G..T..G..G...... 526542 FAM18098 421 CAGGGAAAAATCGTCTACATGAATACGGCTGAAAATAATTTCCTACTTAGCCCTGAACAA 480 FAM17418 79629 ............................................................ 79688 FAM17411 79629 ............................................................ 79688 FAM17422 79629 ............................................................ 79688 FAM13881 253544 ............................................................ 253603 FAM13473 7635 ............................................................ 7576 FAM20559 251659 ............................................................ 251718 DSM 20284 556514 .......C.................................................... 556573 FAM19460 409441 ............................................................ 409500 FAM18987 526543 ..A..........................A.....C..C...T..........G...... 526602 FAM18098 481 TTAGAAAGTACTCTACAAGCGCATCCGCGTGCTAAGGCAATTATTCTCAACTTCCCTTCT 540 FAM17418 79689 ............................................................ 79748 FAM17411 79689 ............................................................ 79748 FAM17422 79689 ............................................................ 79748 FAM13881 253604 ..G......................................................... 253663 FAM13473 7575 ..G......................................................... 7516 FAM20559 251719 ..G......................................................... 251778 DSM 20284 556574 ..G......................................................... 556633 FAM19460 409501 ..G......................................................... 409560 FAM18987 526603 ..G....CC...T.G.....T..C....AA..A...........C..T..T..T.....A 526662 FAM18098 541 AATCCAACGGGGATGACTTACACTAAAGAACAATTGACCGCTTTAGCAGATATTCTGCGA 600 FAM17418 79749 ............................................................ 79808 FAM17411 79749 ............................................................ 79808 FAM17422 79749 ............................................................ 79808 FAM13881 253664 ............................................................ 253723 FAM13473 7515 ............................................................ 7456 FAM20559 251779 ............................................................ 251838 DSM 20284 556634 ....................................G....................... 556693 FAM19460 409561 ....................................G....................... 409620 FAM18987 526663 ..C.................T.....G.....G........AC...........T.A..T 526722 FAM18098 601 AAGTATGATATTTTCGTAATTAGTGATGAAATCTATAGCGACTTGATTTTTGATCAAAAA 660 FAM17418 79809 ............................................................ 79868 FAM17411 79809 ............................................................ 79868 FAM17422 79809 ............................................................ 79868 FAM13881 253724 ............................................................ 253783 FAM13473 7455 ............................................................ 7396 FAM20559 251839 ............................................................ 251898 DSM 20284 556694 ............................................................ 556753 FAM19460 409621 ......................................T..................... 409680 FAM18987 526723 .....C........T........C..C...........T...C.A.....C..C...G.G 526782 FAM18098 661 CACTACTCGATTTCTCATCTATTACCAGATCAAACGATTGTGCTCAACGGCCTTTCTAAG 720 FAM17418 79869 ............................................................ 79928 FAM17411 79869 ............................................................ 79928 FAM17422 79869 ............................................................ 79928 FAM13881 253784 ............................................T............... 253843 FAM13473 7395 ............................................T............... 7336 FAM20559 251899 ............................................T............... 251958 DSM 20284 556754 ............................................................ 556813 FAM19460 409681 ............................................................ 409740 FAM18987 526783 ..............C..C..T.....C..C.....C..C..T..T.....A......... 526842 FAM18098 721 TCACATGCAATGACTGGCTGGCGAGTAGGCATCATTGCCGGACCAGCTCCATTAGTTGAG 780 FAM17418 79929 ............................................................ 79988 FAM17411 79929 ............................................................ 79988 FAM17422 79929 ............................................................ 79988 FAM13881 253844 ............................................................ 253903 FAM13473 7335 ............................................................ 7276 FAM20559 251959 ............................................................ 252018 DSM 20284 556814 ............................................................ 556873 FAM19460 409741 ............................................................ 409800 FAM18987 526843 ..T..C...........T.....G..C..A..T..............C..G.....CC.. 526902 FAM18098 781 CAAATTGAAAAAATTCACGAGCTGTCGGTTACCAGCATTACGACGGTTGCCCAATACGCG 840 FAM17418 79989 ............................................................ 80048 FAM17411 79989 ............................................................ 80048 FAM17422 79989 ............................................................ 80048 FAM13881 253904 ............................................................ 253963 FAM13473 7275 ............................................................ 7216 FAM20559 252019 ............................................................ 252078 DSM 20284 556874 ............................................................ 556933 FAM19460 409801 ............................................................ 409860 FAM18987 526903 ..G...........C..T..AT.A........T.....C..A.................C 526962 FAM18098 841 GCCTTGGAGGCTTTTCAAAACGGGCTCAACGATAGCGAACCGATGCGCGTAGAATACAAA 900 FAM17418 80049 ............................................................ 80108 FAM17411 80049 ............................................................ 80108 FAM17422 80049 ............................................................ 80108 FAM13881 253964 ..............C..............................T.............. 254023 FAM13473 7215 ..............C..............................T.............. 7156 FAM20559 252079 ..............C..............................T.............. 252138 DSM 20284 56934 ..............C............................................. 556993 FAM19460 409861 ..............C...............A............................. 409920 FAM18987 526963 ........A.....C.....T..A..T.........A.............T......C.. 527022 FAM18098 901 AAACGGCGCGATTATTTGCATGATGCCCTCGTTAAGTTGGGGATTGAATGCAGCAAGCCG 960 FAM17418 80109 ............................................................ 80168 FAM17411 80109 ............................................................ 80168 FAM17422 80109 ............................................................ 80168 FAM13881 254024 ............................................................ 254083 FAM13473 7155 ............................................................ 7096 FAM20559 252139 ............................................................ 252198 DSM 20284 56994 ............................................................ 557053 FAM19460 409921 ...............C............................................ 409980 FAM18987 527023 ..G..T..G..C..CC.C..C..C...........A..A..C..C........T..A..A 527082 FAM18098 961 CAAGGTACCTTCTACCTGATGGCTAAAATTCCGAGTGACTTAGAACAAGATGACTTCAAA 1020 FAM17418 80169 ............................................................ 80228 FAM17411 80169 ............................................................ 80228 FAM17422 80169 ............................................................ 80228 FAM13881 254084 .................................G.......................... 254143 FAM13473 7095 .................................G.......................... 7036 FAM20559 252199 .................................G.......................... 252258 DSM 20284 57054 .................A...............G.......................... 557113 FAM19460 409981 .................................G.......................... 410040 FAM18987 527083 ...........T...T.A................................C......... 527142 FAM18098 1021 TTCAGTATGCAACTTGCTAAGGAAGCTAAAGTCGCCGTTATCCCGGGTTCTAGCTTTGGT 1080 FAM17418 80229 ............................................................ 80288 FAM17411 80229 ............................................................ 80288 FAM17422 80229 ............................................................ 80288 FAM13881 254144 ............................................................ 254203 FAM13473 7035 ............................................................ 6976 FAM20559 252259 ............................................................ 252318 DSM 20284 557114 ............................................................ 557173 FAM19460 410041 ............................................................ 410100 FAM18987 527143 ..T........G.................G..T.....C..............T.....C 527202 FAM18098 1081 CCTGGTGGTGAAGGCTACGTTCGCTTTAGCTATGCAGCAAGTATGGACGATCTAACTAAC 1140 FAM17418 80289 ............................................................ 80348 FAM17411 80289 ............................................................ 80348 FAM17422 80289 ............................................................ 80348 FAM18098 606118 ............................................................ 606059 FAM13881 254204 ............................................................ 254263 FAM13473 6975 ............................................................ 6916 FAM20559 252319 ............................................................ 252378 DSM 20284 557174 ............................................................ 557233 FAM19460 410101 ............................................................ 410160 FAM18987 527203 ..C..C........A.....A.....C..T.....G..............CT........ 527262 primer_aat_Rv 3'-GGAGTTTCGATTTGCTCGT-5' FAM18098 1141 GCAATTGCGCGGATTGAAAAATTCCTCAAAGCTAAACGAGCATAA 1185 FAM17418 80349 ............................................. 80393 FAM17411 80349 ............................................. 80393 FAM17422 80349 ............................................. 80393 FAM13881 254264 ..........................T.................. 254308 FAM13473 6915 ..........................T.................. 6871 FAM20559 252379 ..........................T.................. 252423 DSM 20284 557234 ..........................T.................G 557277 FAM19460 410161 ..........................T.................G 410204 FAM18987 527263 ..GG.......A....C.G....T..T...AA............G 527306