Transcriptional profiling and physiological roles of Aedes aegypti spermathecal-related genes
Posted on 2020-03-24 - 13:01
Abstract Background Successful mating of female mosquitoes typically occurs once, with the male sperm being stored in the female spermatheca for every subsequent oviposition event. The female spermatheca is responsible for the maintenance, nourishment, and protection of the male sperm against damage during storage. Aedes aegypti is a major vector of arboviruses, including Yellow Fever, Dengue, Chikungunya, and Zika. Vector control is difficult due to this mosquito high reproductive capacity. Results Following comparative RNA-seq analyses of spermathecae obtained from virgin and inseminated females, eight transcripts were selected based on their putative roles in sperm maintenance and survival, including energy metabolism, chitin components, transcriptional regulation, hormonal signaling, enzymatic activity, antimicrobial activity, and ionic homeostasis. In situ RNA hybridization confirmed tissue-specific expression of the eight transcripts. Following RNA interference (RNAi), observed outcomes varied between targeted transcripts, affecting mosquito survival, egg morphology, fecundity, and sperm motility within the spermathecae. Conclusions This study identified spermatheca-specific transcripts associated with sperm storage in Ae. aegypti. Using RNAi we characterized the role of eight spermathecal transcripts on various aspects of female fecundity and offspring survival. RNAi-induced knockdown of transcript AeSigP-66,427, coding for a Na+/Ca2+ protein exchanger, specifically interfered with egg production and reduced sperm motility. Our results bring new insights into the molecular basis of sperm storage and identify potential targets for Ae. aegypti control.
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Pascini, Tales Vicari; Ramalho-Ortigão, Marcelo; Ribeiro, José Marcos; Jacobs-Lorena, Marcelo; Martins, Gustavo Ferreira (2020). Transcriptional profiling and physiological roles of Aedes aegypti spermathecal-related genes. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4851360.v2
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AUTHORS (5)
TP
Tales Vicari Pascini
MR
Marcelo Ramalho-Ortigão
JR
José Marcos Ribeiro
MJ
Marcelo Jacobs-Lorena
GM
Gustavo Ferreira Martins