Speed dating for enzymes! Finding the perfect phosphopantetheinyl transferase partner for your polyketide synthase
Posted on 2022-01-11 - 04:40
Abstract The biosynthetic pathways for the fungal polyketides bikaverin and bostrycoidin, from Fusarium verticillioides and Fusarium solani respectively, were reconstructed and heterologously expressed in S. cerevisiae alongside seven different phosphopantetheinyl transferases (PPTases) from a variety of origins spanning bacterial, yeast and fungal origins. In order to gauge the efficiency of the interaction between the ACP-domains of the polyketide synthases (PKS) and PPTases, each were co-expressed individually and the resulting production of target polyketides were determined after 48 h of growth. In co-expression with both biosynthetic pathways, the PPTase from Fusarium verticillioides (FvPPT1) proved most efficient at producing both bikaverin and bostrycoidin, at 1.4 mg/L and 5.9 mg/L respectively. Furthermore, the remaining PPTases showed the ability to interact with both PKS’s, except for a single PKS-PPTase combination. The results indicate that it is possible to boost the production of a target polyketide, simply by utilizing a more optimal PPTase partner, instead of the commonly used PPTases; NpgA, Gsp and Sfp, from Aspergillus nidulans, Brevibacillus brevis and Bacillus subtilis respectively.
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Pedersen, Tobias Bruun; Nielsen, Mikkel Rank; Kristensen, Sebastian Birkedal; Spedtsberg, Eva Mie Lang; Sørensen, Trine; Petersen, Celine; et al. (2022): Speed dating for enzymes! Finding the perfect phosphopantetheinyl transferase partner for your polyketide synthase. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.5786722.v1
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Tobias Bruun Pedersen
Mikkel Rank Nielsen
Sebastian Birkedal Kristensen
Eva Mie Lang Spedtsberg
Teis Esben Sondergaard
Kåre Lehmann Nielsen
Donald Max Gardiner
Jens Laurids Sørensen
bacillus subtilis respectivelyorigins spanning bacterialremaining pptases showedpolyketide synthase abstractcommonly used pptasesfusarium solani respectivelyoptimal pptase partnerfungal polyketides bikaverinfungal originstarget polyketidesfusarium verticillioidestarget polyketidepolyketide synthasesl respectivelyspeed datingresults indicatepptase combinationheterologously expressedexpressed individuallybrevibacillus brevisbiosynthetic pathwaysaspergillus nidulans9 mg48 h4 mg