Roles of hsa-miR-12462 and SLC9A1 in acute myeloid leukemia
Posted on 2020-07-24 - 03:58
Abstract MicroRNAs (miRNAs) play important roles in cell proliferation, differentiation, and survival and may be useful for acute myeloid leukemia (AML) diagnosis and prognosis. In this study, we defined a novel miRNA, hsa-miR-12462, through small RNA sequencing of the bone marrow (BM) cells from 128 AML patients. Overexpression of hsa-miR-12462 in AML cells (U937 and HL-60) significantly decreased their growth rate when compared with those of the wild-type and MOCK controls. In a xenograft mouse model, tumor weight and size in the mice bearing the U937 cells with hsa-miR-12462 overexpression were significantly reduced when compared with those bearing the mock cells. The AML cells overexpressing hsa-miR-12462 had increased sensitivity to cytarabine chemotherapy. Combining the data from the MiRDB, an online microRNA database ( http://mirdb.org ), with the RNA-sequencing results, SLC9A1 was predicted to be one of the targets of hsa-miR-12462. hsa-miR-12462 was further confirmed to bind exclusively to the 3′UTR of SLC9A1 in U937 cells, leading to downregulation of SLC9A1. In summary, a higher level of hsa-miR-12462 in AML cells is associated with increased sensitivity to cytarabine chemotherapy via downregulation of SLC9A1.
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Jia, Yan; Liu, Wei; Zhan, Hui-En; Yi, Xiao-Ping; Liang, Hui; Zheng, Qi-Li; et al. (2020). Roles of hsa-miR-12462 and SLC9A1 in acute myeloid leukemia. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.5072003.v1
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AUTHORS (11)
YJ
Yan Jia
WL
Wei Liu
HZ
Hui-En Zhan
XY
Xiao-Ping Yi
HL
Hui Liang
QZ
Qi-Li Zheng
XJ
Xin-Ya Jiang
HZ
Hai-Yan Zhou
LZ
Liang Zhao
XZ
Xie-Lan Zhao
HZ
Hui Zeng