Rapid and visual detection of milk vetch dwarf virus using recombinase polymerase amplification combined with lateral flow strips
Version 2 2020-08-01, 07:13
Version 1 2020-07-12, 04:18
Posted on 2020-08-01 - 07:13
Abstract Background Milk vetch dwarf virus (MDV) is an important ssDNA virus which causes yellowing, stunting and leaf rolling symptoms on legumes. In China, the virus causes great economic losses and has recently been found to infect tobacco. The expansion of its host range and its ability to spread rapidly has given rise to the urgent need for a sensitive, specific and rapid diagnostic assay that can assist in effective disease control. Methods Assays based on the polymerase chain reaction combined with lateral flow strip detection (PCR-LFS) and recombinase polymerase amplification combined with LFS (RPA-LFS) were developed targeting the coat protein (CP) gene of MDV. Results The PCR and RPA assays could detect respectively 103 copies or 101 copies of MDV by agarose gel electrophoresis. The PCR-LFS and RPA-LFS assays developed could both detect as few as 101 copies per reaction at 37 °C. Both methods could detect MDV in crude leaf extracts. Conclusions The RPA-LFS assay developed is a rapid, sensitive and specific method for detecting MDV, which is convenient and has great potential for use in the field.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Cao, Yuhao; Yan, Dankan; Wu, Xinyang; Chen, Ziqiang; Lai, Yuchao; Lv, Lanqing; et al. (2020). Rapid and visual detection of milk vetch dwarf virus using recombinase polymerase amplification combined with lateral flow strips. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.5057023.v2
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (10)
YC
Yuhao Cao
DY
Dankan Yan
XW
Xinyang Wu
ZC
Ziqiang Chen
YL
Yuchao Lai
LL
Lanqing Lv
FY
Fei Yan
JC
Jianping Chen
HZ
Hongying Zheng
XS
Xuemei Song