RNA G-quadruplex structures exist and function in vivo in plants
Posted on 2020-09-02 - 03:44
Abstract Background Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct evidence for RNA G-quadruplex formation in living eukaryotic cells. Therefore, it is unclear whether any purported functions are associated with the specific sequence content or the formation of an RNA G-quadruplex structure. Results Using rG4-seq, we profile the landscape of those guanine-rich regions with the in vitro folding potential in the Arabidopsis transcriptome. We find a global enrichment of RNA G-quadruplexes with two G-quartets whereby the folding potential is strongly influenced by RNA secondary structures. Using in vitro and in vivo RNA chemical structure profiling, we determine that hundreds of RNA G-quadruplex structures are strongly folded in both Arabidopsis and rice, providing direct evidence of RNA G-quadruplex formation in living eukaryotic cells. Subsequent genetic and biochemical analyses show that RNA G-quadruplex folding is able to regulate translation and modulate plant growth. Conclusions Our study reveals the existence of RNA G-quadruplex in vivo and indicates that RNA G-quadruplex structures act as important regulators of plant development and growth.
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Yang, Xiaofei; Cheema, Jitender; Zhang, Yueying; Deng, Hongjing; Duncan, Susan; Umar, Mubarak Ishaq; et al. (2020). RNA G-quadruplex structures exist and function in vivo in plants. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.5109708.v1Â
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AUTHORS (11)
XY
Xiaofei Yang
JC
Jitender Cheema
YZ
Yueying Zhang
HD
Hongjing Deng
SD
Susan Duncan
MU
Mubarak Ishaq Umar
JZ
Jieyu Zhao
QL
Qi Liu
XC
Xiaofeng Cao
CK
Chun Kit Kwok
YD
Yiliang Ding