Preliminary report of a novel formulation of clinical-grade, fully matured, tumor-associated peptide-loaded dendritic cells for cancer immunotherapy
Posted on 2019-11-18 - 04:18
Abstract Background We developed and clinically test a new method to manufacture mature dendritic cells for autologous cell therapy of solid and hematological malignancies. Methods Peripheral blood monocytes are matured into dendritic cells with GM-CSF/IL-4 mixture, and then activated through IL1b, TNFa, IFNa-2A, and Poly(I:C), then viably frozen until use. DC are injected intra-dermally for a total of 6 injections. Results High-grade mDC with high pre- and post- freezing yields could be generated. These DC spontaneously produce IL12p70 and they can be further stimulated via CD40. In a patient with advanced pancreatic cancer treated with our mDC formulation, we observed no toxicity but remarkable immune response and objective response in terms of tumor shrinking. Conclusion We describe a new method to manufacture GMP-grade dendritic cells for autologous therapeutic cancer vaccines. We also show proof-of-principle efficacy in a patient with advanced pancreatic cancer. Trial registration NCT02705703.
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Mirandola, Leonardo; Chiriva-Internati, Maurizio; Bresalier, Robert; Marincola, Francesco; Figueroa, Jose; Dahlbeck, Scott (2019). Preliminary report of a novel formulation of clinical-grade, fully matured, tumor-associated peptide-loaded dendritic cells for cancer immunotherapy. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4742594.v1
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AUTHORS (6)
LM
Leonardo Mirandola
MC
Maurizio Chiriva-Internati
RB
Robert Bresalier
FM
Francesco Marincola
JF
Jose Figueroa
SD
Scott Dahlbeck