Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities
Posted on 2020-04-28 - 04:22
Abstract Eugenia punicifolia (Kunth) D. C. (Myrtaceae) has been showing interesting biological activities in the literature which was correlated to its phenolic compounds. In the sense of a better recovering of phenolics with the best antioxidant and antiproliferative activities, an extraction, based on multivariate analytical approach, was developed from E. punicifolia leaves. The different extractor solvents (ethanol, methanol and water) and their binary and ternary combinations were evaluated using a simplex-centroid mixture design and surface response methodology. The optimized crude extracts were investigated for phenol and flavonoid content and compared to their antioxidant (EC50) and antiproliferative properties against HEp-2 (cell line derived from the oropharyngeal carcinoma) and mononuclear viability cells. Ethanolic extracts showed the best phenolic content with the highest antioxidant activity and moderated activity antiproliferative to HEp-2. ESI-QTOF–MS revealed the presence of quercetin and myricetin derivatives, which was correlated to activities tested. Then, simplex-centroid design allowed us to correlate the Eugenia punicifolia biological activities with the extracts obtained from solvent different polarity mixtures.
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dos Santos, Catarina; Mizobucchi, Andressa Lie; Escaramboni, Bruna; Lopes, Bruno Pereira; Angolini, Celio Fernando Figueiredo; Eberlin, Marcos Nogueira; et al. (2020). Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4955141.v1
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AUTHORS (8)
Cd
Catarina dos Santos
AM
Andressa Lie Mizobucchi
BE
Bruna Escaramboni
BL
Bruno Pereira Lopes
CA
Celio Fernando Figueiredo Angolini
ME
Marcos Nogueira Eberlin
Kd
Karina Alves de Toledo
EN
Eutimio Gustavo Fernández Núñez