N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping
Posted on 2019-09-08 - 03:55
Abstract Background N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research. The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protein function. However, these studies focused mainly on the analysis of specific sample type, and lack the integration of glycoproteomic data from different tissues, body fluids or cell types. Methods In this study, we collected the human glycosite-containing peptides identified through their de-glycosylated forms by mass spectrometry from over 100 publications and unpublished datasets generated from our laboratory. A database resource termed N-GlycositeAtlas was created and further used for the distribution analyses of glycoproteins among different human cells, tissues and body fluids. Finally, a web interface of N-GlycositeAtlas was created to maximize the utility and value of the database. Results The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representingâ>â14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies. Conclusions The entire human N-glycoproteome database as well as 22 sub-databases associated with individual tissues or body fluids can be downloaded from the N-GlycositeAtlas website at http://nglycositeatlas.biomarkercenter.org .
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Sun, Shisheng; Hu, Yingwei; Ao, Minghui; Shah, Punit; Chen, Jing; Yang, Weiming; et al. (2019). N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4658060.v1
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AUTHORS (10)
SS
Shisheng Sun
YH
Yingwei Hu
MA
Minghui Ao
PS
Punit Shah
JC
Jing Chen
WY
Weiming Yang
XJ
Xingwang Jia
YT
Yuan Tian
ST
Stefani Thomas
HZ
Hui Zhang