Springer Nature
Browse

Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer

Posted on 2019-01-30 - 05:00
Abstract Objectives To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. Results The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression.

CITE THIS COLLECTION

DataCite
No result found
or
Select your citation style and then place your mouse over the citation text to select it.

SHARE

email

Usage metrics

BMC Research Notes

AUTHORS (17)

  • Pere Puig
    Nadina Erill
    Marta Terricabras
    Isaac Subirana
    Judit González-García
    Adrià Asensi-Puig
    Michael Donovan
    Lourdes Mengual
    M. Agulló-Ortuño
    Mireia Olivan
    Antonio Alcaraz
    José López-Martín
    Inés Torres
    José Rodríguez-Peralto
    Alfredo Rodríguez-Antolín
    Juan Morote
    Víctor González-Rumayor
need help?