Mesenchymal stem cells alleviate liver injury induced by chronic-binge ethanol feeding in mice via release of TSG6 and suppression of STAT3 activation
Posted on 2020-01-14 - 05:16
Abstract Background Mesenchymal stem cells (MSCs) are a population of pluripotent cells that might be used for treatment of liver disease. However, the efficacy of MSCs for mice with alcoholic hepatitis (AH) and its underlying mechanism remains unclear. Methods MSCs were isolated from the bone marrow (BM) of 4–6-week-old male C57BL/6 N mice. AH was induced in female mice by chronic-binge ethanol feeding for 10 days. The mice were given intraperitoneal injections of MSCs with or without transfection or AG490, recombinant mouse tumor necrosis factor (TNF)-α-stimulated gene/protein 6 (rmTSG-6), or saline at day 10. Blood samples and hepatic tissues were collected at day 11. Various assays such as biochemistry, histology, and flow cytometry were performed. Results MSCs reduced AH in mice, decreasing liver/body weight ratio, liver injury, blood and hepatic lipids, malondialdehyde, interleukin (IL)-6, and TNF-ɑ, but increasing glutathione, IL-10, and TSG-6, compared to control mice. Few MSCs engrafted into the inflamed liver. Knockdown of TSG-6 in MSCs significantly attenuated their effects, and injection of rmTSG-6 achieved similar effects to MSCs. The signal transducer and activator of transcription 3 (STAT3) was activated in mice with AH, and MSCs and rmTSG-6 inhibited the STAT3 activation. Injection of MSCs plus AG490 obtained more alleviation of liver injury than MSCs alone. Conclusions BM-MSCs injected into mice with AH do not engraft the liver, but they secrete TSG-6 to reduce liver injury and to inhibit STAT3 activation.
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Wan, Yue-Meng; Li, Zhi-qiang; Zhou, Qiong; Liu, Chang; Wang, Men-Jie; Wu, Hui-Xin; et al. (2020). Mesenchymal stem cells alleviate liver injury induced by chronic-binge ethanol feeding in mice via release of TSG6 and suppression of STAT3 activation. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4816206.v1
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AUTHORS (16)
YW
Yue-Meng Wan
ZL
Zhi-qiang Li
QZ
Qiong Zhou
CL
Chang Liu
MW
Men-Jie Wang
HW
Hui-Xin Wu
YM
Yun-Zhen Mu
YH
Yue-Feng He
YZ
Yuan Zhang
XW
Xi-Nan Wu
YL
Yu-Hua Li
ZX
Zhi-Yuan Xu
HW
Hua-Mei Wu
YX
Ying Xu
JY
Jin-Hui Yang
XW
Xiao-Fang Wang