Long noncoding RNA Smyca coactivates TGF-β/Smad and Myc pathways to drive tumor progression
Posted on 2022-07-26 - 11:50
Abstract Background Metastasis and chemoresistance are major culprits of cancer mortality, but factors contributing to these processes are incompletely understood. Methods Bioinformatics methods were used to identify the relations of Smyca expression to clinicopathological features of human cancers. RNA-sequencing analysis was used to reveal Smyca-regulated transcriptome. RNA pull-down and RNA immunoprecipitation were used to examine the binding of Smyca to Smad3/4 and c-Myc/Max. Chromatin immunoprecipitation and chromatin isolation by RNA purification were used to determine the binding of transcription factors and Smyca to various gene loci, respectively. Real-time RT-PCR and luciferase assay were used to examine gene expression levels and promoter activities, respectively. Xenograft mouse models were performed to evaluate the effects of Smyca on metastasis and chemoresistance. Nanoparticle-assisted gapmer antisense oligonucleotides delivery was used to target Smyca in vivo. Results We identify lncRNA Smyca for its association with poor prognosis of many cancer types. Smyca potentiates metabolic reprogramming, migration, invasion, cancer stemness, metastasis and chemoresistance. Mechanistically, Smyca enhances TGF-β/Smad signaling by acting as a scaffold for promoting Smad3/Smad4 association and further serves as a Smad target to amplify/prolong TGF-β signaling. Additionally, Smyca potentiates c-Myc-mediated transcription by enhancing the recruitment of c-Myc/Max complex to a set of target promoters and c-Myc binding to TRRAP. Through potentiating TGF-β and c-Myc pathways, Smyca synergizes the Warburg effect elicited by both pathways but evades the anti-proliferative effect of TGF-β. Targeting Smyca prevents metastasis and overcomes chemoresistance. Conclusions This study uncovers a lncRNA that coordinates tumor-relevant pathways to orchestra a pro-tumor program and establishes the clinical values of Smyca in cancer prognosis and therapy.
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Chen, Hsin-Yi; Chan, Shu-Jou; Liu, Xinxin; Wei, An-Chi; Jian, Ru-In; Huang, Kuan-Wei; et al. (2022). Long noncoding RNA Smyca coactivates TGF-β/Smad and Myc pathways to drive tumor progression. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.6082528.v1
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AUTHORS (16)
HC
Hsin-Yi Chen
SC
Shu-Jou Chan
XL
Xinxin Liu
AW
An-Chi Wei
RJ
Ru-In Jian
KH
Kuan-Wei Huang
YL
Yaw-Dong Lang
JS
Jou-Ho Shih
CL
Chun-Chieh Liao
CL
Chiu-Lin Luan
YK
Yu-Tung Kao
SC
Shang-Yin Chiang
PH
Pei-Wen Hsiao
YJ
Yuh-Shan Jou
YC
Yunching Chen
RC
Ruey-Hwa Chen