Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412
Posted on 2019-07-28 - 03:44
Abstract Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the peptide mass fingerprinting matched an uncharacterized protein (WP_017418614.1), which was then named AMEP412. AMEP412 could cause a clearly defined HR necrosis in tobacco leaves, which was less affected by thermal treatment. The sub-cellular localization assay revealed that AMEP412 localized on the cell surface. This protein could also trigger early defense events such as the generation of reactive oxygen species (H2O2 and O2â) and the induction of defense enzymes, including superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL). Moreover, AMEP412 could stimulate plant systemic resistance against Pseudomonas syringae pv. tomato DC3000.
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Shen, Yongrui; Li, Jianwei; Xiang, Junliang; Wang, Jiaqi; Yin, Kuide; Liu, Quan (2019). Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4591808.v1
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AUTHORS (6)
YS
Yongrui Shen
JL
Jianwei Li
JX
Junliang Xiang
JW
Jiaqi Wang
KY
Kuide Yin
QL
Quan Liu