Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside
Posted on 2020-07-15 - 04:36
Abstract We report the high production of stilbenes, including resveratrol and viniferin, in grapevine (Vitis labruscana L.) cell cultures through elicitation with methyl jasmonate (MeJA) and stevioside (STE). Methyl-β-cyclodextrin (MeβCD) is widely used as a solubilizer for resveratrol production. For the first time, we used STE as a solubilizer for stilbene production in plant cell cultures. MeJA was most effective elicitor in activating VvSTS expression and stimulating stilbene biosynthesis in grapevine cell cultures. The maximum concentration of δ-viniferin (892.2 mg/L) production with a small amount of trans-resveratrol (12.2 mg/L) was observed in the culture medium by co-treatment of cells with MeJA and STE, whereas the highest level of trans-resveratrol (371.9 mg/L) with a slight amount of δ-viniferin (11.5 mg/L) was accumulated in the culture medium of cells treated with MeJA and MeβCD. However, neither trans-resveratrol nor δ-viniferin were significantly elevated within the cells by the applications. Notably, predominant production of δ-viniferin and trans-resveratrol was observed in shake and static flask culture medium, respectively, by co-treatment of MeJA and STE. Furthermore, stilbene compounds of resveratrol, ε-viniferin, and δ-viniferin were mainly produced in a 3-L bioreactor culture following elicitation of cells with MeJA and STE. These results provide new strategies for conditional, high-level production of resveratrol and viniferin in cell cultures by utilizing the solubilizing properties of STE or MeβCD.
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Jeong, Yu Jeong; Park, Su Hyun; Park, Sung-Chul; Kim, Soyoung; Kim, Tae Hee; Lee, Jiyoung; et al. (2020). Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.5059648.v1
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AUTHORS (10)
YJ
Yu Jeong Jeong
SP
Su Hyun Park
SP
Sung-Chul Park
SK
Soyoung Kim
TK
Tae Hee Kim
JL
Jiyoung Lee
SK
Suk Weon Kim
YR
Young Bae Ryu
JJ
Jae Cheol Jeong
CK
Cha Young Kim