Gapless assembly of maize chromosomes using long-read technologies
Posted on 2020-05-21 - 03:47
Abstract Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. This genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), and 53 Mb of the Ab10 meiotic drive haplotype. The data also reveal the internal structure of seven centromeres and five heterochromatic knobs, showing that the major tandem repeat arrays (CentC, knob180, and TR-1) are discontinuous and frequently interspersed with retroelements.
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Liu, Jianing; Seetharam, Arun S.; Chougule, Kapeel; Ou, Shujun; Swentowsky, Kyle W.; Gent, Jonathan I.; et al. (2020). Gapless assembly of maize chromosomes using long-read technologies. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4986905.v1
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AUTHORS (18)
JL
Jianing Liu
AS
Arun S. Seetharam
KC
Kapeel Chougule
SO
Shujun Ou
KS
Kyle W. Swentowsky
JG
Jonathan I. Gent
VL
Victor Llaca
MW
Margaret R. Woodhouse
NM
Nancy Manchanda
GP
Gernot G. Presting
DK
David A. Kudrna
MA
Magdy Alabady
CH
Candice N. Hirsch
KF
Kevin A. Fengler
DW
Doreen Ware
TM
Todd P. Michael
MH
Matthew B. Hufford
RD
R. Kelly Dawe