Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population
Posted on 2023-04-13 - 11:25
Abstract Background Rapid determination of an individual’s antibody status can be beneficial in understanding an individual’s immune response to SARS-CoV-2 and for initiation of therapies that are only deemed effective in sero-negative individuals. Antibody lateral flow tests (LFTs) have potential to address this need as a rapid, point of care test. Methods Here we present a proof-of-concept evaluation of eight LFT brands using sera from 95 vaccinated individuals to determine sensitivity for detecting vaccination generated antibodies. Samples were analysed on eight different brands of antibody LFT and an automated chemiluminescent microparticle immunoassay (CMIA) that identifies anti-spike antibodies which was used as our reference standard. Results All 95 (100%) participants tested positive for anti-spike antibodies by the chemiluminescent microparticle immunoassay (CMIA) reference standard post-dose two of their SARS-CoV-2 vaccine: BNT162b2 (Pfizer/BioNTech, n = 60), AZD1222 (AstraZeneca, n = 31), mRNA-1273 (Moderna, n = 2) and Undeclared Vaccine Brand (n = 2). Sensitivity increased from dose one to dose two in six out of eight LFTs with three tests achieving 100% sensitivity at dose two in detecting anti-spike antibodies. Conclusions These tests are demonstrated to be highly sensitive to detect raised antibody levels in vaccinated individuals. RDTs are low cost and rapid alternatives to ELISA based systems.
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Greenland-Bews, Caitlin; Byrne, Rachel L.; Owen, Sophie I.; Watkins, Rachel L.; Bengey, Daisy; Buist, Kate; et al. (2023). Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.6582902.v1
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AUTHORS (22)
CG
Caitlin Greenland-Bews
RB
Rachel L. Byrne
SO
Sophie I. Owen
RW
Rachel L. Watkins
DB
Daisy Bengey
KB
Kate Buist
KC
Karina Clerkin
CE
Camille Escadafal
LF
Lorna S. Finch
SG
Susan Gould
EG
Emanuele Giorgi
AH
Andy Hodgkinson
LM
Larysa Mashenko
DP
Darren Powell
HS
Helen R. Savage
CT
Caitlin R. Thompson
LT
Lance Turtle
JW
Jahanara Wardale
DW
Dominic Wooding
TE
Thomas Edwards