Epigenetic silencing of the ANKRD26 gene correlates to the pro-inflammatory profile and increased cardio-metabolic risk factors in human obesity
Posted on 2019-12-05 - 05:29
Abstract Background Obesity is a major worldwide threat to human health. Increasing evidence indicates that epigenetic modifications have a major impact on the natural history of this disorder. Ankyrin Repeat Domain 26 (Ankrd26) is involved in the development of both obesity and diabetes in mice and is modulated by environmentally induced epigenetic modifications. This study aims at investigating whether impaired ANKRD26 gene expression and methylation occur in human obesity and whether they correlate to the phenotype of these subjects. Results We found that downregulation of ANKRD26 mRNA and hyper-methylation of a specific region of the ANKRD26 promoter, embedding the CpG dinucleotides − 689, − 659, and − 651 bp, occur in peripheral blood leukocytes from obese compared with the lean subjects. ANKRD26 gene expression correlates inversely to the percentage of DNA methylation at these 3 CpG sites. Luciferase assays reveal a cause-effect relationship between DNA methylation at the 3 CpG sites and ANKRD26 gene expression. Finally, both ANKRD26 mRNA levels and CpG methylation correlate to body mass index and to the pro-inflammatory status and the increased cardio-metabolic risk factors of these same subjects. Conclusion Downregulation of the ANKRD26 gene and hyper-methylation at specific CpGs of its promoter are common abnormalities in obese patients. These changes correlate to the pro-inflammatory profile and the cardio-metabolic risk factors of the obese individuals, indicating that, in humans, they mark adverse health outcomes.
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Desiderio, Antonella; Longo, Michele; Parrillo, Luca; Campitelli, Michele; Cacace, Giuseppe; Simone, Sonia; et al. (2019). Epigenetic silencing of the ANKRD26 gene correlates to the pro-inflammatory profile and increased cardio-metabolic risk factors in human obesity. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4770368.v1
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AUTHORS (15)
AD
Antonella Desiderio
ML
Michele Longo
LP
Luca Parrillo
MC
Michele Campitelli
GC
Giuseppe Cacace
SS
Sonia Simone
RS
Rosa Spinelli
FZ
Federica Zatterale
SC
Serena Cabaro
PD
Pasquale Dolce
PF
Pietro Formisano
MM
Marco Milone
CM
Claudia Miele
FB
Francesco Beguinot
GR
Gregory Raciti