Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation
Posted on 2019-10-13 - 12:34
Abstract Background The epigenetic machinery is frequently altered in acute myeloid leukemia. Focusing on cytogenetically normal (CN) AML, we previously described an abnormal H3K27me3 enrichment covering 70 kb on the HIST1 cluster (6.p22) in CN-AML patient blasts. Here, we further investigate the molecular, functional, and prognosis significance of this epigenetic alteration named H3K27me3 HIST1 in NPM1-mutated (NPM1mut) CN-AML. Results We found that three quarter of the NPM1mut CN-AML patients were H3K27me3 HIST1high. H3K27me3 HIST1high group of patients was associated with a favorable outcome independently of known molecular risk factors. In gene expression profiling, the H3K27me3 HIST1high mark was associated with lower expression of the histone genes HIST1H1D, HIST1H2BG, HIST1H2AE, and HIST1H3F and an upregulation of genes involved in myelomonocytic differentiation. Mass spectrometry analyses confirmed that the linker histone protein H1d, but not the other histone H1 subtypes, was downregulated in the H3K27me3 HIST1high group of patients. H1d knockdown primed ATRA-mediated differentiation of OCI-AML3 and U937 AML cell lines, as assessed on CD11b/CD11c markers, morphological and gene expression analyses. Conclusions Our data suggest that NPM1mut AML prognosis depends on the epigenetic silencing of the HIST1 cluster and that, among the H3K27me3 silenced histone genes, HIST1H1D plays a role in AML blast differentiation. Graphical abstract
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Garciaz, Sylvain; N’guyen Dasi, Lia; Finetti, Pascal; Chevalier, Christine; Vernerey, Julien; Poplineau, Mathilde; et al. (2019). Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4696703.v1
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AUTHORS (17)
SG
Sylvain Garciaz
LN
Lia N’guyen Dasi
PF
Pascal Finetti
CC
Christine Chevalier
JV
Julien Vernerey
MP
Mathilde Poplineau
NP
Nadine Platet
SA
Stéphane Audebert
MP
Matthieu Pophillat
LC
Luc Camoin
FB
François Bertucci
BC
Boris Calmels
CR
Christian Récher
DB
Daniel Birnbaum
CC
Christian Chabannon
NV
Norbert Vey
ED
Estelle Duprez