Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H2O2

Published on 2019-06-17T05:00:00Z (GMT) by
Abstract Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H2O2. Apoptosis of HBE-Dp71AS cells induced by H2O2 was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinase B (p-AKT) were detected in the HBE-Dp71AS cells, which functioned together with RAD51 as the molecular explanations for the character alterations of HBE-Dp71AS cells.

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Tan, Sichuang; Zhao, Shuai; Xiao, Xuefei; Xiao, Lan; Xie, Jinliang; Tan, Sipin (2019): Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H2O2. figshare. Collection.