Determination of reliable lung function parameters in intubated mice
Posted on 2019-09-15 - 03:38
Abstract Background Animal models and, in particular, mice models, are important tools to investigate the pathogenesis of respiratory diseases and to test potential new therapeutic drugs. Lung function measurement is a key step in such investigation. In mice, it is usually performed using forced oscillation technique (FOT), negative pressure-driven forced expiratory (NPFE) and pressure-volume (PV) curve maneuvers. However, these techniques require a tracheostomy, which therefore only allows end-point measurements. Orotracheal intubation has been reported to be feasible and to give reproducible lung function measurements, but the agreement between intubation and tracheostomy generated-data remains to be tested. Methods Using the Flexivent system, we measured lung function parameters (in particular, forced vital capacity (FVC), forced expiratory volume in the first 0.1 s (FEV0.1), compliance (Crs) of the respiratory system, compliance (C) measured using PV loop and an estimate of inspiratory capacity (A)) in healthy intubated BALB/cJ mice and C57BL/6 J mice and compared the results with similar measurements performed in the same mice subsequently tracheostomized after intubation, by means of paired comparison method, correlation and Bland-Altman analysis. The feasibility of repetitive lung function measurements by intubation was also tested. Results We identified parameters that are accurately evaluated in intubated animals (i.e., FVC, FEV0.1, Crs, C and A in BALB/cJ and FVC, FEV0.1, and A in C57BL/6 J). Repetitive lung function measurements were obtained in C57BL/6 J mice. Conclusion This subset of lung function parameters in orotracheally intubated mice is reliable, thereby allowing relevant longitudinal studies.
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Bonnardel, Eline; Prevel, Renaud; Campagnac, Marilyne; Dubreuil, Marielle; Marthan, Roger; Berger, Patrick; et al. (2019). Determination of reliable lung function parameters in intubated mice. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4666580.v1
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AUTHORS (7)
EB
Eline Bonnardel
RP
Renaud Prevel
MC
Marilyne Campagnac
MD
Marielle Dubreuil
RM
Roger Marthan
PB
Patrick Berger
ID
Isabelle Dupin