Data set containing live cell microscopy videos, uncropped western blots and images of a proximity-ligation assay corresponding to the article: Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress.

This collection contains three datasets corresponding to the above mentioned manuscript by Jochner et al., which investigates the role of phosphatase and tensin homolog long (PTEN-L) in mouse primary neurons in the context of cerebral ischemia. PTEN-L is a longer translational variant of PTEN, which antagonises an important cell survival pathway and might have neuroprotective properties in neurons.

After ischemic-like stress, PTEN-L rapidly re-distributes within neuronal cells while the shorter variant PTEN remains evenly distributed within neurons (Data Set 1: Live cell microscopy videos of cellular distribution of PTEN:EGFP and PTEN-L:EGFP after 50µM glutamate stress in mouse primary neurons). Here, primary neurons derived from conditional PTEN knockout mice were transduced with cre to knock both PTEN variants and exogenous, EGFP-tagged PTEN-L or PTEN protein was expressed. Neurons were imaged every 10 min for 90 min after they were treated with 50 µM glutamate. This Data corresponds to Fig. 3a-b of the above mentioned publication.

Data Set 2 (Uncropped Western Blots, Fig. 1b and Fig. 2b) contains uncropped Western Blots of Fig. 1b and Fig. 2b, which showed the knock-out of both PTEN variants and the titration of exogenous PTEN protein amounts.

Data Set 3 (Proximity ligation assay of PTEN-L:EGFP (V420) with Gab2 after control (PBS) or 50µM glutamate treatment) contains the original images used to quantify the proximity ligation-assay corresponding to Fig. 4c. PTEN-L interacts with the membrane-associated protein Gab2 and the interaction is lost in response to treatment with 50 µM glutamate.