12934_2019_1071_MOESM3_ESM.pptx (504.5 kB)
MOESM3 of Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein
presentation
posted on 2019-01-29, 05:00 authored by Isabel Guerrero Montero, Katarzyna Dolata, Rabea Schlüter, Gilles Malherbe, Susanne Sievers, Daniela Zühlke, Thomas Sura, Emma Dave, Katharina Riedel, Colin RobinsonAdditional file 3: Figure S1. The swarming motilities of E. coli CyDisCo strains and P. aeruginosa. A swarming motility assay was performed using E. coli CyDisCo with an empty plasmid; expressing scFv; expressing misfolded scFv (mf_scFv) and P. aeruginosa PA01 as a positive control. Swarming plates (0.3% agar in LB medium) were spotted with 10 µL of overnight cultures and incubated for 24 h at 37 °C. After 24 h the E. coli CyDisCo strains did not show signs of cell swarming, while P. aeruginosa showed a positive swarming phenotype. Assays were performed in triplicate. Figure shows representative results from 24 h incubation.