13071_2020_4241_MOESM5_ESM.pptx (243.42 kB)
Additional file 5 of Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP4)
presentation
posted on 2020-07-23, 04:53 authored by Mona S. Mahmoud, Omnia M. Kandil, Nadia T. Abu El-Ezz, Seham H. M. Hendawy, Bassma S. M. Elsawy, Donald P. Knowles, Reginaldo G. Bastos, Lowell S. Kappmeyer, Jacob M. Laughery, Heba F. Alzan, Carlos E. SuarezAdditional file 5: Figure S4. a Anti-histidin antibodies recognize a 37 kDa antigen in a cell lysate of recombinant E. coli transformed with TOPO® TA plasmid containing the full-length Bcsbp4 gene. Lane 1: (+ ve) cultures induced with L-Arabinose; Lane 2: (- ve) non-Arabinose induced cultures. b Specific recognition of rBcSBP4 protein by B. caballi positive serum in ELISA (a 1:100 dilution of serum was serially diluted by two-fold) using: serum from an equid derived from a horse experimentally infected with B. caballi (blue columns); field horse positive for B. caballi infection (orange columns); and horse that tested negative to infection with B. caballi (grey columns).
