posted on 2016-08-22, 05:00authored byBiswanath Chatterjee, David Wolff, Mathivanan Jothi, Munmun Mal, Asoke Mal
Expression of HA-tagged MKK6EE and MKK6DN in C2C12 cells was verified by western blot analysis, probed with antibodies against HA, and β-actin as loading control. (B) Control IgG or anti-phospho-p38 immunoprecipitates retrieved from extracts of indicated cells grown in GM or DM were subjected to in vitro kinase assays to monitor p38 activation using GST-ATF2 as substrate. Autoradiography and Commassie detected phosphorylated ATF2 and GST-ATF2 protein, respectively. (PPTX 75 kb)
Funding
National Institute of Arthritis and Musculoskeletal and Skin Diseases