Additional file 4: of The association of genomic lesions and PD-1/PD-L1 expression in resected triple-negative breast cancers
presentation
posted on 2018-07-11, 05:00 authored by Michael Barrett, Elizabeth Lenkiewicz, Smriti Malasi, Anamika Basu, Jennifer Yearley, Lakshmanan Annamalai, Ann McCullough, Heidi Kosiorek, Pooja Narang, Melissa Wilson Sayres, Meixuan Chen, Karen Anderson, Barbara PockajFigure S4. FISH validation of high-level 9p24.1 amplicon. A) DNA content histogram of flow-sorted TNBC-11. B) Chromosome 9 Comparative Genomic Hybridization plot with high-level (log2 ratio >4) gain of JAK2 locus (arrow) at 9p24.1. C) Multi-color FISH assay [5′JAK2[9p24](green)/ 3′JAK2[9p24](red)/CEN 9(aqua)] image indicates more than 21 intact JAK2 signals and 1–3 CEN 9 signals. Abbreviations: FISH fluorescence in situ hybridization, TNBC triple-negative breast cancer. (PPTX 948 kb)
