Springer Nature
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Additional file 4: Figure S4. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

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posted on 2016-12-13, 05:00 authored by Christopher Penton, Vasudeo Badarinarayana, Joy Prisco, Elaine Powers, Mark Pincus, Ronald Allen, Paul August
Laminin 521 promotes increased mdx/BL10 differentiation. Cells were freshly isolated from male mdx/BL10 limb muscles and plated on multiple matrices: laminin 111, laminin 211, laminin 521, and growth factor reduced Matrigel. MHC area quantification shows a trend towards increased differentiation on laminin 521 compared to other substrates. N = 3 technical replicates per substrate. Differences were not found to be statistically different by one-way ANOVA and Bonferroni correction.


Muscular Dystrophy Association