Additional file 3 of ROS-dependent HIF1α activation under forced lipid catabolism entails glycolysis and mitophagy as mediators of higher proliferation rate in cervical cancer cells

Additional file 1 Fig. S3. Me-180 cells were transfected with ATGL plasmid for 48 h. (A) Western blot analysis of HIF1α, BNIP3 levels. Band intensity is indicated below the corresponding band and expressed as fold-change relative to CTRL. The images are representative of three independent experiments that gave similar results. β-Actin and ATGL were used as loading and transfection control, respectively. (B) Me-180 cells, transfected as previously described, were treated 24 h before the end of experiment with 5 mM NAC. Band intensity is indicated below the corresponding band and expressed as fold-change relative to CTRL. The image of Western blot analysis of HIF1α, BNIP3 levels, is representative of three independent experiments that gave similar results. β-Actin and ATGL were used as loading and transfection control, respectively.