Additional file 3: Figure S3. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion
presentationposted on 2016-12-13, 05:00 authored by Christopher Penton, Vasudeo Badarinarayana, Joy Prisco, Elaine Powers, Mark Pincus, Ronald Allen, Paul August
Laminin 521 promotes increased human myogenic cell proliferation. Cells were expanded on laminin 111, laminin 211, laminin 521, or growth factor reduced Matrigel for 5 passages. For proliferation analysis, cells were seeded at a density of approximately 1500 cells per well and incubated for 2Â days. Afterwards, cells were fixed and nuclei counts were performed using Hoechst staining (Life Technologies). Nâ=â5 technical replicates. Significance was determined by one-way ANOVA with Bonferroni correction. Significance is annotated as less than .0001 (****) with laminin 521 as control.