Additional file 3: Figure S2. of A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression
presentation
posted on 2016-10-18, 05:00 authored by Makoto Kawaguchi, Noboru Hara, Vladimir Bilim, Hiroshi Koike, Mituko Suzuki, Tae-Sun Kim, Nan Gao, Yu Dong, Sheng Zhang, Yuji Fujinawa, Osamu Yamamoto, Hiromi Ito, Yoshihiko Tomita, Yuchi Naruse, Akira Sakamaki, Yoko Ishii, Koichi Tsuneyama, Masaaki Inoue, Johbu Itoh, Masanori Yasuda, Nobuo Sakata, Cha-Gyun Jung, Satoshi Kanazawa, Hiroyasu Akatsu, Hiroshi Minato, Takayuki Nojima, Kiyofumi Asai, Yutaka MiuraA, T24 cells showed the most malignant staining pattern of ATBF1 (Fig. 2B) and HT1376 showed the most benign staining pattern (Fig. 2B) similar to the staining pattern of RT4. RT4 is a cell line derived from non-malignant papilloma. Scale bar = 5 μm. B, Western blot analysis of D1-120 showed the loss of ATBF1 in T24 cells. The result is relevant to the pathological staining with D1-120 regarding the loss of ATBF1 in the nucleus and cytoplasm. RT-4 and HT1376 cells expressed smaller fragments of ATBF1 in the cytoplasm and larger fragments of ATBF1 in the nucleus. The results suggest that the ATBF1 in the cytoplasm was not the full length but fragments of ATBF1. C, T24 cells, which expressed no ATBF1 in the nucleus, grew faster than the other two cell lines expressing ATBF1 in the nucleus. (PPTX 493 kb)
