Additional file 2 of ROS-dependent HIF1α activation under forced lipid catabolism entails glycolysis and mitophagy as mediators of higher proliferation rate in cervical cancer cells

Additional file 2 Fig. S2. (A) Proliferation rate of HeLa cells over-expressing ATGL and BNIP3 ΔTM was assayed by Trypan blue direct cell counting procedure. Data are expressed as mean ± SD (n = 3; * p < 0.05; ** p < 0.01 as indicated). (B) Flow cytometry analyses of apoptosis induction in HeLa cells after 48 h of ATGL and BNIP3 ΔTM over-expression, by using Annexin-V and propidium iodide (PI) fluorescence staining assay. Each scatter plot shows the percentage of early apoptotic cells (Annexin-V + cells, lower right quadrant) and late apoptotic cells (PI + and Annexin V + cells, upper right quadrant).