13046_2021_1832_MOESM2_ESM.pptx (60.4 kB)
Additional file 2 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma
presentation
posted on 2021-01-19, 04:39 authored by Aurore Dumond, Etienne Brachet, Jérôme Durivault, Valérie Vial, Anna K. Puszko, Yves Lepelletier, Christopher Montemagno, Marina Pagnuzzi-Boncompagni, Olivier Hermine, Christiane Garbay, Nathalie Lagarde, Matthieu Montes, Luc Demange, Renaud Grépin, Gilles PagèsAdditional file 2: Fig. S1. Study of down-regulation of NRPs by shRNA in 786-O cells. (A-B) Effects of the downregulation of NRP by shRNA on NRP1 and NRP2 mRNA expression measured by qPCR. (C) Effects on cell metabolic activity measured by MTT assays. (D) Down-regulation of NRPs decreased cell migration. Bevacizumab increased this effect for NRP1 down-regulation. (E) Down-regulation of NRPs had no effect on VEGFA and VEGFC production measured by ELISA. *p < 0.05; **p < 0.01; *** p < 0.001.
