Additional file 2 of Histone tail analysis reveals H3K36me2 and H4K16ac as epigenetic signatures of diffuse intrinsic pontine glioma

Additional file 2: S2. Targeted mass spectrometry for histone tail analysis. Overview of study design and analysis of histone tail post-translational modification states in pediatric glioma specimens. Tumor tissue specimens were obtained post-mortem or during the course of treatment. Patient-derived tumor cell lines were established from tissue specimens. Extracted histones were analyzed using targeted mass spectrometry of the histone H3 and H4 N-terminal tail, with quantitation of histone post-translational modifications (me1, me2, me3, ac) and unmodified peptide on lysine (K) residues.