12896_2018_416_MOESM2_ESM.pptx (1.83 MB)

Additional file 2: of Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

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posted on 17.02.2018, 05:00 by Paola Mini, Olivia Demurtas, Silvia Valentini, Patrizia Pallara, Giuseppe Aprea, Paola Ferrante, Giovanni Giuliano
Figure S2. Retention of the Luc transgene in a set of Chlamydomonas colonies transformed with the pAgroLucR plasmid. cw15 cells were co-cultivated with the Agrobacterium C58C1 strain harboring the pAgroLucR plasmid. The DNA extracted was analyzed for the presence of the genes shown in Additional file 1: Figure S1 plus the Luc gene. The vast majority of the transformants, although positive for the presence of the Paro gene, do not contain an intact Luc gene. A and B are two independent control transformants, positive for the presence of the Paro and Luc genes. P: pAgroLucR plasmid. C-: negative control. M: 1 Kb Plus DNA Ladder (Life Technologies). Oligonucleotide sequences are shown in Additional file 7: Table S1. (PPTX 1875 kb)


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