Additional file 2: of Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study

Figure S2. Retention of the Luc transgene in a set of Chlamydomonas colonies transformed with the pAgroLucR plasmid. cw15 cells were co-cultivated with the Agrobacterium C58C1 strain harboring the pAgroLucR plasmid. The DNA extracted was analyzed for the presence of the genes shown in Additional file 1: Figure S1 plus the Luc gene. The vast majority of the transformants, although positive for the presence of the Paro gene, do not contain an intact Luc gene. A and B are two independent control transformants, positive for the presence of the Paro and Luc genes. P: pAgroLucR plasmid. C-: negative control. M: 1 Kb Plus DNA Ladder (Life Technologies). Oligonucleotide sequences are shown in Additional file 7: Table S1. (PPTX 1875 kb)