13048_2016_235_MOESM2_ESM.ppt (100.5 kB)

Additional file 2: Figure S2. of Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines

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posted on 18.04.2016, 05:00 by Ahmed Abdelbaset-Ismail, Daniel Pedziwiatr, Ewa Suszyńska, Sylwia Sluczanowska-Glabowska, Gabriela Schneider, Sham Kakar, Mariusz Ratajczak
1,25-dihydroxyvitamin D3 induces proliferation of quiescent BM-derived very small embryonic-like stem cells (VSELs) in vivo. Panel A. After treatment with 1,25-dihydroxyvitamin D3, as shown here, no significant difference in BrdU-incorporated HSCs was observed in treated and control animals (6 male mice/group). Panel B. BrdU incorporation into VSELs after 1,25-dihydroxyvitamin D3 treatment. The percentages of VSELs that incorporated BrdU into newly synthesized DNA. After treatment with 1,25-dihydroxyvitamin D3 at 800 IU/dose, ~13.2 % of VSELs were BrdU+, in contrast to the control group in which ~7 % of VSELs were BrdU+ (6 male mice/group). The incorporation of BrdU into HSCs and VSELs was measured by FACS. (PPT 100 kb)


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