13045_2017_421_MOESM2_ESM.pptx (396.84 kB)
Additional file 2: Figure S1. of Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways
presentation
posted on 2017-02-28, 05:00 authored by Yan-Jiun Huang, Ching-Kuo Yang, Po-Li Wei, Thanh-Tuan Huynh, Jacqueline Whang-Peng, Tzu-Ching Meng, Michael Hsiao, Yew-Ming Tzeng, Alexander Wu, Yun YenYAP1 overexpression (OE) leads to increased stemness in colon cancer cells. (A) A significantly increased CD133+ cell percentage was found in the YAP1 OE cells as compared to their wild-type counterparts as demonstrated by our flow cytometric analysis. (B) YAP1-overexpressing HCT116 and DLD-1 cells were found to form a significantly higher number of tumor spheres (*P ≤ 0.05, **P ≤ 0.01). (C) Comparative western blots of wild-type and YAP1 overexpressing HCT116 and DLD-1 cells. An increased expression of β-catenin, NF-kB, vimentin, and Kras is associated with YAP1 overexpression. (PPTX 396 kb)
