Additional file 1: of Trophoblast differentiation, invasion and hormone secretion in a three-dimensional in vitro implantation model with rhesus monkey embryos

Schematic illustration of rhesus macaque embryo culture in the Matrigel-feeder cell 3-D in vitro implantation system. The Matrigel carrier was gelled onto a coverslip then placed into a 35 mm well containing feeder layer of Buffalo Rat Liver cells, followed by injection of one rhesus macaque blastocyst stage embryo into each Matrigel carrier. (PPTX 41 kb)